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AAV6 VP3, Recombinant Protein

分子别名(Synonym)

VP3

表达区间及表达系统(Source)

AAV6 VP3, Recombinant Protein (VP3-A5146) is expressed from E. coli cells. It contains AA Met 203 - Leu 736 (Accession # O56137-1).

Predicted N-terminus: Met

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 61.6 kDa. The protein migrates as 58-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 6 M Urea, PBS, pH7.3 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

VP3 SDS-PAGE

AAV6 VP3, Recombinant Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

 

背景(Background)

Adeno-associated virus is a single-stranded DNA virus and the current scientific consensus is that it does not cause any human disease. It consists of a protein capsid (CAPside) and a 4.7 KB length single stranded DNA genome. The protein capsid consists of three subunits, VP1, VP2, and VP3.

 

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