膜杰作
Star Staining
分子别名（Synonym）
B220, CD45, CD45R, GP180, IMD105, L-CA, LCA, LY5, T200
表达区间及表达系统（Source）
Human CD45RO Protein, His Tag (CDO-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Lys 416 (Accession # P08575-4).
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蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 46.4 kDa. The protein migrates as 70-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human CD45RO Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human CD45RO Protein, His Tag (Cat. No. CDO-H52H3) is more than 85% and the molecular weight of this protein is around 80-105 kDa verified by SEC-MALS.
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背景（Background）
CD45RO contains exon 3, 7 and 8 and lacks the RA, RB and RC exons of the CD45 gene. CD45 is a receptor protein tyrosine phosphatase, also known as Ly-5 or leukocyte common antigen. CD45 mainly involves in the initiation of T cell receptor signaling by controlling the activation of the Src family protein-tyrosine kinases Lck and Fyn. CD45 deficiency causes in T- and B-lymphocyte dysfunction in the form of severe combined immune deficiency. It also takes a significant role in autoimmune diseases and cancer as well as in infectious diseases including fungal infections.
