膜杰作
Star Staining
抗体来源（Source）
HRP conjugated Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1G11) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
1M1G11
种属（Species）
Rabbit
亚型（Isotype）
Rabbit IgG | Rabbit Kappa
偶联（Conjugate）
HRP-Conjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Chemical
特异性（Specificity）
This product is a specific antibody specifically reacts with Eribulin.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.5-250 ng/mL |
纯度（Purity）
>90% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
HRP conjugated Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1G11) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Farletuzumab Ecteribulin at 1 μg/mL (100 μL/well) can bind HRP conjugated Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1G11) (Cat. No. ERN-PLM12b) with a linear range of 0.5-16 ng/mL (QC tested).
Protocol
Immobilized Human FOLR1, Fc Tag (Cat. No. FO1-H5253) at 1 μg/mL, add Farletuzumab Ecteribulin in the 100% Human Serum and then add HRP conjugated Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1G11) (Cat. No. ERN-PLM12b) at 0.05 μg/mL (QC tested).
Protocol
背景（Background）
Eribulin is a synthetic analogue of the macrocyclic polyether halichondrin B, which was originally isolated from the Asian sea sponge Halichondria okadai. Eribulin binds specifically to the β-tubulin subunit on the (+) end of the microtubule and potently inhibits elongation of the formed microtubule, while having little or no effect on microtubule depolymerization. Eribulin's potent antimitotic activity and nonmitotic effects on tumor biology make it an interesting candidate for investigation as a MTA payload for ADCs.
