膜杰作
Star Staining
产品描述（Product Details）
Human iPSC-Derived Dopamine Neurons are specialized cells capable of mimicking the properties of human dopamine neurons, crucial for various neurological studies. Our Human iPSC-derived dopamine neurons (Parkinson's disease) are generated from human induced pluripotent stem cells (iPSCs) using a fully defined, proprietary differentiation protocol.
These neurons are thoroughly characterized through immunocytochemistry, targeting well-known markers such as TH, and MAP2, ensuring their identity and functionality. They have been validated for their ability to function in electrophysiological assays and disease modeling.
Additionally, our dopamine neurons are cryopreserved at an optimal stage of differentiation, guaranteeing their functionality and ability to be used directly from thaw. This ensures a consistent and reliable cell source for research on Parkinson's disease and other neurological disorders.
产品特征（Product Specification）
Cryopreserved cells, shipped on dry ice. Each vial contains over 1 x 10^6 cells.
存储（Storage）
Frozen in liquid nitrogen.
验证数据（Validation Data）
Functional Activity
Spontaneous Network Burst Activity
This data set highlights the intrinsic activity of our Human iPSC-Derived Dopamine Neurons (Cat. No. CIPC-DDC001) cultured on an MEA plate. The neurons exhibit robust spontaneous firing, as visualized in the accompanying heatmap video. The raster plot clearly shows regular network burst firing patterns, indicative of well-coordinated neuronal activity. The photo of the neurons on the MEA plate further confirms the successful culture and network formation, making this data a powerful demonstration of their functional connectivity and suitability for neurophysiological studies.
Haloperidol-Induced Modulation of Neuronal Firing
This data set demonstrates the dose-dependent effects of Haloperidol on neuronal firing. Raster plots and associated firing parameters (for Weighted Mean Firing Rate, Number of Bursts, Burst Duration, Burst Frequency, and Number of Network Bursts, n = 2) are presented for varying Haloperidol concentrations. At 0.1 μM, the firing activity is enhanced, while at 1 μM, the activity diminishes. At 10 μM, the firing is nearly abolished. These results are consistent with findings from Yokoi et al. (2019), where Haloperidol is known to inhibit D2 receptors at low doses and 5-HT2 receptors at high doses (Tyler et al., 2017). This confirms that the relevant receptors in our Human iPSC-Derived Dopamine Neurons (Cat. No. CIPC-DDC001) are functioning normally, underscoring their utility in drug screening and neurotoxicity studies.
