膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| EP168-C01 | High-bind Plate | 1 plate |
| EP168-C02 | Human DR3 | 100 μg |
| EP168-C03 | Human TL1A-SABC | 2.5μg |
| EP168-C04 | Human Anti-TL1A Neutralizing Antibody | 100 μg |
| EP168-C05 | Coating Buffer | 12mL |
| EP168-C06 | 20xWashing Buffer | 50mL |
| EP168-C07 | Blocking Buffer | 50mL |
| EP168-C08 | Substrate Solution | 12mL |
| EP168-C09 | Stop Solution | 7mL |
产品概述（Product Overview）
TNF-like cytokine 1A (TL1A) and its receptors, death receptor 3 (DR3) and decoy receptor 3 (DcR3) are members of the TNF and TNF receptor superfamilies of proteins, respectively. Binding of APC-derived TL1A to lymphocytic DR3 provides co-stimulatory signals for activated lymphocytes. DR3 signaling affects not only the proliferative activity of and cytokine production by effector lymphocytes, but also critically influences the development and suppressive function of regulatory T-cells. Whereas, DcR3 restricts the function of the TL1A/DR3 complex: attenuating T-cell activation and downregulating the secretion of pro-inflammatory cytokines. Together with DR3 and DcR3, TL1A constitutes a cytokine system that actively interferes with the regulation of immune responses.
应用说明（Application）
This kit is developed for screening for inhibitors of human TL1A binding to human DR3.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
2-8℃
质量管理控制体系（QMS）
典型数据-Typical Data Please refer to DS document for the assay protocol.
Serial dilutions of Human Anti-TL1A Neutralizing antibody (Catalog # EP168-C04) (1:1 serial dilution, from 5 μg/mL to0.0390625 μg/mL) was added into Human DR3 : Biotinylated Human TL1A binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The example data is for reference only.
产品推荐（Recommended Products）
背景（Background）：TL1A-DR3
The TL1A protein, belongs to the tumor necrosis factor (TNF) family and is a receptor for TNFRSF25 and TNFRSF6B. TL1A is involved in the activation of NF-κB and C-Jun pathways, which can be used as a regulator of mucosal immunity and participate in the immune pathway of inflammatory bowel disease (IBD) pathogenesis.
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