分子别名(Synonym)
MATA2, MATII, SAMS2
表达区间及表达系统(Source)
Human MAT2A Protein, His Tag (MAA-H51H3) is expressed from E. coli cells. It contains AA Asn 2 - Tyr 395 (Accession # P31153-1).
Predicted N-terminus: Met
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 45.5 kDa. The protein migrates as 45-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with glycerol as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳(SDS-PAGE)
Human MAT2A Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性(Bioactivity)
Measured via hydrolysis of ATP during synthesis of S-adenosylmethionine. The specific activity is >200 pmol/min/μg (QC tested).
Protocol
背景(Background)
The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes. [provided by RefSeq, Jun 2011]