膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| RAS203-C01 | Pre-coated Anti-RSV-Pre-Fusion glycoprotein F0 (site IV) Antibody Microplate | 1 plate |
| RAS203-C02 | Pre-Fusion glycoprotein F0 (RSV) Standard | 15 μg |
| RAS203-C03 | HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody | 20 μg |
| RAS203-C04 | 10×Washing Buffer | 50 mL |
| RAS203-C05 | 2×Dilution Buffer | 50 mL |
| RAS203-C06 | Substrate Solution | 12 mL |
| RAS203-C07 | Stop Solution | 7 mL |
背景（Background）
Respiratory syncytial virus (RSV) is a highly contagious virus causing severe infection in infants and the elderly. Various approaches are being used to develop an effective RSV vaccine. The RSV fusion (F) subunit, particularly the cleaved trimeric pre-fusion F, is one of the most promising vaccine candidates under development.
A rapid and effective assay kit detecting the levels of HRSV Pre-Fusion glycoprotein F0 is urgently needed to accelerate the development of RSV vaccines.
应用说明（Application）
The kit has been tested to specifically identify HRSV (A) and HRSV (B) Pre-Fusion glycoprotein. It was developed for the specific quantitative detection of HRSV Pre-Fusion glycoprotein F0 (site Ⅳ) in samples.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of HRSV Pre-Fusion glycoprotein F0 (site Ⅳ). The kit consists of Pre-coated Anti-RSV-Pre-Fusion glycoprotein F0 (site Ⅳ) Antibody Microplate and Pre-Fusion glycoprotein F0 (RSV) Standard,HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody and buffers.
Your experiment will include 5 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the Pre-Fusion glycoprotein F0 (RSV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add the HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
