膜杰作
Star Staining
分子别名（Synonym）
BLR2, CC-CKR-7, CCR-7, CD197, CDw197, CMKBR7, EBI1
表达区间及表达系统（Source）
Human CCR7 Full Length Protein, His,Flag Tag (CC7-H52D3) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Pro 378 (Accession # P32248).
Predicted N-terminus: Gln 25
Request for sequence
蛋白结构（Molecular Characterization）
The protein has a calculated MW of 58.9 kDa.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>85% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of LMNG.
LMNG and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove LMNG and CHS.
LMNG/CHS buffer (LG-13) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, LMNG, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
活性（Bioactivity）-ELISA
Immobilized Human CCR7 Full Length Protein, His,Flag Tag (Cat. No. CC7-H52D3) at 1 μg/mL (100 μL/well) can bind Anti-CCR7 Antibody, Human IgG1 with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Anti-CCR7 Antibody, Human IgG1 captured on Protein A Chip can bind Human CCR7 Full Length Protein, His,Flag Tag (Cat. No. CC7-H52D3) with an affinity constant of 121 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
The monoclonal antibody mAb-729 reacts with the human CC chemokine receptor, CCR7. The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV)，and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. Signals mediated by this receptor regulate T cell homeostasis in lymph nodes, and may also function in the activation and polarization of T cells, and in chronic inflammation pathogenesis.
