膜杰作
Star Staining
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The HEK293/Human IDH1(132R)-P2A-mGFP&Luc Stable Cell Line was engineered to not only express the receptor full length human IDH1 (Gene ID: 3417) and mGFP by a viral P2A self-cleaving peptide, but also express the firefly luciferase reporter. The expression of mGFP characterizing the human IDH1 (132R) was confirmed by flow cytometry. The Luciferase activity was confirmed by the detection of luminescence signal.
应用说明（Application）
• Screen for human IDH1 (132R) inhibitor
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (20 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of human IDH1 (132R) on HEK293/Human IDH1(132R)-P2A-mGFP&Luc Stable Cell Line by FACS.
The expression of mGFP characterizing the human IDH1 (132R) was detected by flow cytometry.
Protocol
Application
Expression analysis of Luciferase on HEK293/Human IDH1(132R)-P2A-mGFP&Luc Stable Cell Line (RLU).
The luminescence signal of HEK293/Human IDH1(132R)-P2A-mGFP&Luc Stable Cell Line were detected by incubating with the luciferase substrate. The RLU value was approximately 5.89 x10^7 at the density of 1x10^4 cells/well.
Protocol
Luciferase activity of HEK293/Human IDH1(132R)-P2A-mGFP&Luc Stable Cell Line (FOLD).
The luminescence signal of HEK293/Human IDH1(132R)-P2A-mGFP&Luc Stable Cell Line were detected by incubating with the luciferase substrate. The induction fold was approximately 7917.73.
Protocol
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Limited Use&License Disclosure
- This cell line is provided for research use only. It is neither intended for any animal or human therapeutic use nor for any direct human in vivo use. You are restricted to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order.cn@acrobiosystems.com for further details.
