膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
CD71
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Recombinant Human CD71 is expressed from human 293 cells.
特异性（Specificity）
Specifically recognizes Human CD71.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-63 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
This product No cross-reactivity in ELISA with
Rat Transferrin R, His Tag (Cat. No. TFR-R5249).
Mouse Transferrin R, His Tag (Cat. No. TFR-M524b).
电泳（SDS-PAGE）
Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Cynomolgus Transferrin R, His Tag (Cat. No. TFR-C524a) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
Immobilized Human Transferrin R, His Tag (Cat. No. CD1-H5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Captured Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) on CM5 chip via anti-mouse antibodies surface can bind Human Transferrin R, His Tag (Cat. No. CD1-H5243) with an affinity constant of 0.821 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.
