Influenza B/Victoria Lineage Viruses Hemagglutinin (HA) Specific ELISA Kit

组分（Materials Provided）

背景（Background）

Influenza, commonly known as 'the flu', is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenzavirus A, Influenzavirus B, and Influenzavirus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle which makes it a great target for vaccine studies.

应用说明（Application）

This kit is developed for specific quantitative detection of influenza B/Victoria lineage viruses HA protein in samples.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Influenza B/Victoria lineage viruses HA. The kit consists of Pre-coated Anti-HA (Influenza B/Victoria Lineage) Antibody Microplate and HA (Influenza B/Victoria Lineage) Standard,HRP-Anti-HA (Influenza B/Victoria Lineage) Antibody and buffers.

Your experiment will include 5 simple steps:

a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

b) Add your sample to the plate, take the HA (Influenza B/Victoria Lineage) as Control sample. The samples and Control sample are diluted by Dilution Buffer.

c) Add the HRP-Anti-HA (Influenza B/Victoria Lineage) Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add TMB.

e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.