膜杰作
Star Staining
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracking record, stringent quality control and validated cell passage stability
- Parent cell lines obtained legally from internationally renowned cell collection organizations
- Commercial license available for regulatory filing
描述（Description）
The Human TACI (Luc) HEK293 Reporter Cell was engineered to not only express NF-κB signaling response element, but also express the receptors full length human TACI (Gene ID: 23495). When stimulated with human APRIL or BAFF protein, receptor-mediated signaling can drive NF-κB-mediated luminescence. Neutralization of biological effect of the ligand-receptor interaction by corresponding antibody or fusion protein results in a decrease in luminescence.
应用说明（Application）
• Screen for neutralizing antibodies or fusion proteins blocking the stimulation of human APRIL or BAFF protein.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (20 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Application
Inhibition of human APRIL protein-induced reporter activity by human BAFF and APRIL dual inhibitor.
The Human TACI (Luc) HEK293 Reporter Cell was incubated with serial dilutions of inhibitors in the presence of human APRIL protein (Cat. No. APL-H52D1) with a final concentration of 0.1 μg/mL. The EC50 of human BAFF and APRIL dual inhibitor (Telitacicept) is approximately 1.452 μg/mL.
Protocol
Inhibition of human BAFF protein-induced reporter activity by human BAFF and APRIL dual inhibitor.
The Human TACI (Luc) HEK293 Reporter Cell was incubated with serial dilutions of inhibitors in the presence of human BAFF protein (Cat. No. BAF-H52D4) with a final concentration of 0.3 μg/mL. The EC50 of human BAFF and APRIL dual inhibitor (Telitacicept) is approximately 0.1998 μg/mL.
Protocol
Signaling Bioassay
Response to human APRIL protein (RLU).
The Human TACI (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human APRIL protein (Cat. No. APL-H52D1). The EC50 was approximately 0.1169 μg/mL.
Protocol
Response to human APRIL protein (FOLD).
The Human TACI (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human APRIL protein (Cat. No. APL-H52D1). The max induction fold was approximately 11.81.
Protocol
Response to human BAFF protein (RLU).
The Human TACI (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human BAFF protein (Cat. No. BAF-H52D4). The EC50 was approximately 0.2826 μg/mL.
Protocol
Response to human BAFF protein (FOLD).
The Human TACI (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human BAFF protein (Cat. No. BAF-H52D4). The max induction fold was approximately 13.74.
Protocol
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Limited Use&License Disclosure
- This cell line is provided for research use only. It is neither intended for any animal or human therapeutic use nor for any direct human in vivo use. You are restricted to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order.cn@acrobiosystems.com for further details.
