膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Influenza A virus (A/Victoria/4897/2022) NA (H1N1) Antibody, Human IgG1 (7E7) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
7E7
种属（Species）
Mouse
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
virus
免疫原（Immunogen）
Recombinant Influenza A [A/Victoria/4897/2022(H1N1)] Neuraminidase (NA) Protein is expressed from human 293 cells.
特异性（Specificity）
Specifically recognizes Influenza A [A/Victoria/4897/2022(H1N1)] Neuraminidase (NA), Has cross-reactivity in ELISA with: Influenza A [Victoria/2570/2019(H1N1)] Neuraminidase (NA).
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.06-500 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
交叉验证（Cross Verification）
This product No cross-reactivity in ELISA with
Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5245).
Influenza B [PHUKET/3073/2013] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5246).
Influenza A [Darwin/6/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5247).
Influenza A [A/Darwin/9/2021 (H3N2)] Neuraminidase (NA) Protein, His Tag (Cat. No. NE2-V5249).
Influenza A [turkey/Germany-MV/R2472/2014(H5N8)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5249).
Influenza A [Guangdong/18SF020(H5N6)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524h).
Influenza A [Wisconsin/588/2019(H1N1)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524k).
Influenza A [A/Thailand/1(KAN-1)/2004(H5N1)] Neuraminidase (NA) Protein, His Tag (Cat. No. HA1-V5245).
电泳（SDS-PAGE）
Monoclonal Anti-Influenza A virus (A/Victoria/4897/2022) NA (H1N1) Antibody, Human IgG1 (7E7) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Influenza A virus (A/Victoria/4897/2022) NA (H1N1) Antibody, Human IgG1 (7E7) (Cat. No. NE1-MY2148) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Influenza A [A/Victoria/4897/2022(H1N1)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524c) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A virus (A/Victoria/4897/2022) NA (H1N1) Antibody, Human IgG1 (7E7) (Cat. No. NE1-MY2148) with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Immobilized Influenza A [Sydney/5/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5248), Influenza A [Victoria/2570/2019(H1N1)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524e), Influenza A [Wisconsin/67/2022(H1N1)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524m) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A virus (A/Victoria/4897/2022) NA (H1N1) Antibody, Human IgG1 (7E7) (Cat. No. NE1-MY2148) with a linear range of 0.2-125 ng/mL (Routinely tested).
Protocol
- 845XXXXXXX
- Thank you so much for being accommodating and sending the protein to us as fast a possible. We really needed this for an important experiment and I appreciate how well your customer service handled my order. The reply for the invoice went very smoothly as well due to receiving it the same day as the order. I will definitely look for supplies through your company in the future. Overall, I would give Acro Biosystems for my unique protein needs in the future. Thank you.
>- 2021-9-27
- 301XXXXXXX
- My peptides arrived in a timely manner and I had no issues using them in my COVID assay. I would purchase again from ACRO due to their great product quality and customer service. The team was very helpful in answering any questions I had about the product and assured me if any issues did come about I could reach out anytime.
- 2021-9-19
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背景（Background）
Influenza, commonly known as 'the flu', is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenzavirus A, Influenzavirus B, and Influenzavirus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle which makes it a great target for vaccine studies.
