膜杰作
Star Staining
背景（Background）
Amyloid beta 42 ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Amyloid beta 42 in cerebrospinal fluid, serum, plasma or other biological fluids. An antibody specific for Amyloid beta 42 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Amyloid beta 42 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Amyloid beta 42 Antibody, which also binds the Amyloid beta 42 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Amyloid beta 42 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Amyloid beta 42 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Amyloid beta 42 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Amyloid beta 42 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
应用说明（Application）
The kit is developed for the detection and quantitative determination of Amyloid beta 42 in human cerebrospinal fluid or peripheral blood and cell culture supernates.
It is for research use only.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
背景（Background）：Amyloid beta 42
Aggregation of amyloid β (Aβ) plays a key role in the pathogenesis of Alzheimer's disease (AD). Aβ is a proteolytic product of amyloid precursor protein by β- and γ-secretases. The imprecise cleavage of γ-secretase at C-terminus of Aβ sequence results in two major Aβ isoforms: Aβ42 (42 residues long) and Aβ40 (40 residues long). The only difference between Aβ42 and Aβ40 is the two additional C-terminal residues on Aβ42. The concentration of Aβ40 in cerebral spinal fluid has been found to be several-fold more than that of Aβ42. However, Aβ42 is the major component of amyloid plaques in AD brains, while Aβ40 is detected only in a subset of plaques .
