Human Phospho Tau (Thr181) ELISA Kit

背景（Background）

Human Phospho Tau (Thr181) ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human pTau181 in cerebrospinal fluid, serum, plasma or other biological fluids. An antibody specific for pTau181 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the pTau181 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-pTau181 Antibody, which also binds the pTau181 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-pTau181 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-pTau181 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of pTau181 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of pTau181 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

应用说明（Application）

The kit is developed for the detection and quantitative determination of pTau181 in human cerebrospinal fluid or peripheral blood and cell culture supernates.

It is for research use only.

存储（Storage）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.