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APC-Labeled Monoclonal Anti-Human CD2 Antibody, Mouse IgG1 (RPA-2.10) (0.03% Proclin)

相关产品(Related Products): PE

抗体来源(Source)

Monoclonal Anti-Human CD2 Antibody, Mouse IgG1 (RPA-2.10) is a mouse monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.

应用(Application)

Flow Cytometry (Evaluation of the expression of CD2 on Human cells).

克隆号(Clone)

RPA-2.10

亚型(Isotype)

Mouse IgG1 | Mouse Kappa

特异性(Specificity)

This product is a specific antibody specifically reacts with CD19 protein.

种属反应性(Reactivity)

Human

免疫原(Immunogen)

Purified Human CD2 Protein.

偶联(Conjugate)

APC

Excitation Wavelength: 640 nm

Emission Wavelength: 661 nm

推荐稀释比(Recommended Dilution)

1:50

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin 300 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 24 months in lyophilized state;
  2. -70°C for 12 months after reconstitution.
  3. 2-8 °C for 12 months after reconstitution.

质量管理控制体系(QMS)

  1. 质量管理体系(ISO, GMP)
  2. 质量优势
  3. 质控流程
 

活性(Bioactivity)-FACS

CD2 FACS

Flow cytometric analysis of Human peripheral blood lymphocytes staining with APC-Labeled Monoclonal Anti-Human CD2 Antibody, Mouse IgG1 (RPA-2.10) (Cat. No. CD2-AHFM8) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 PBMCs in a final volume of 100 µL), compared with APC Mouse IgG1, κ Isotype Ctrl Antibody. APC signal was used to evaluate the binding activity (QC tested).

Protocol

 
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背景(Background)

T-cell surface antigen CD2 is also known as Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5 and SRBC, is a single-pass type I membrane protein found on the surface of T cells and natural killer (NK) cells. CD2 is a member of the immunoglobulin superfamily. CD2 / SRBC contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD2 / SRBC interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3 / CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. In addition to its adhesive properties, CD2 also acts as a co-stimulatory molecule on T and NK cells. CD2 is a specific marker for T cells and NK cells, and can therefore be used in immunohistochemistry to identify the presence of such cells in tissue sections.

 

前沿进展

Preliminary Investigation into the Use of Amino-Acid-Derived Ionic Liquids for Extracting Cellulose from Waste Biomass to Prepare Cellulose Aerogel Adsorbents
Deng, Zhao, Nian et al
Gels (2025) 11 (3)
Abstract: To investigate the feasibility of cellulose extraction from lignocellulosic waste biomass using ionic liquids-a sustainable and efficient approach-for preparing cellulose aerogel adsorbents, we employed a fully green amino acid-derived ionic liquid, cysteine nitrate ([Cys][NO3]), for cellulose separation from diverse biomass sources. The extracted cellulose, with a purity range of 83.8-93.9%, was processed into cellulose aerogels (CAs) via a conventional aerogel preparation protocol. The resulting CA exhibited promising adsorption capacities, including 0.2-11.6 mg/g for Na+, 4.4-19.9 mg/g for Ca2+, 4.15-35.6 mg/g for Mg2+, and 1.85-13.3 mg/g for Cd2+, as well as 9.7-17.7 g/g for engine oil. These results demonstrate the presence of effective mass transfer channels in the CA, proving that the cellulose's fibrillation capacity was preserved in the pre-treatment. This study illuminates the potential of this green, straightforward method for preparing aerogels from cellulose derived from waste biomass, with promising applications in wastewater treatment and material recovery.
Structural diversity dependent cation incorporation into magnetic Cr-Se nanocrystals
Wang, Zhao, Wang et al
Nanoscale (2025)
Abstract: Chromium selenide (Cr-Se)-based low-dimensional materials have attracted significant attention in spintronics because of their diverse structure- and composition-dependent magnetic properties. While significant progress has been made in fabricating the Cr-Se family of nanomaterials through techniques like chemical vapor deposition, the synthesis of Cr-Se nanocrystals (NCs) via colloidal methods remains underexplored. In this work, we demonstrate the robust colloidal synthesis approach for producing Cr2Se3 and Cr3Se4 NCs with distinct morphologies by varying the Cr precursors and ligands. Cr-Se NCs can serve as templates for cation exchange (CE) reactions involving monovalent Cu+ and Ag+, divalent Zn2+ and Cd2+, and trivalent In3+, facilitating the creation of a diverse library of metal selenide NCs and nanoheterostructures. Our findings highlight how the outcomes of CE reactions are influenced by the structure of various Cr-Se phases. Furthermore, the magnetic properties of the as-synthesized Cr-Se NCs and their derivative CuCrSe2 were investigated. Our work provides a robust synthesis route for the Cr-Se class of magnetic nanomaterials and a platform for creating a diverse range of functional metal selenide NCs via CE reactions.
Generation and characterization of a tamoxifen-inducible lineage tracing tool Cd2-P2A-CreERT2 knock-in mice
Guo, Zhu, Yu et al
Front Immunol (2025) 16, 1482070
Abstract: The new targeted gene editing technologies, such as the CRISPR/Cas system, enable researchers to insert or delete genes at targeted loci efficiently. The Cre-loxp recombination system is widely used to activate or inactivate genes with high spatial and temporal specificity.Using the CRISPR/Cas9 system, we inserted the CreERT2 transgene expression cassette into the Cd2 gene locus to generate conditional Cre-driver line Cd2-CreERT2 knock-in mice, which drove the expression of CreERT2 by the endogenous Cd2 promoter. By mating the Cd2-CreERT2 strain with a Rosa26-LSL-tdTomato reporter mouse strain which contains a tdTomato expression fragment blocked with a loxP-flanked STOP cassette (LSL) driven by a CAG promoter, a Cd2-CreERT2;Rosa26-LSL-tdTomato reporter strain was obtained to evaluate the expression pattern of CD2 in different cell types.After treatment with tamoxifen, the Cd2-CreERT2 knock-in mice were induced to perform efficient recombination at the loxP site following CreERT2 activation and cause the expression of tdTomato fluorescence. The tdTomato and CD2 were expressed in the T cells of peripheral blood, spleen and mesenteric lymph nodes, whereas detected in a low proportion in the B cells. While about 20% of cells labeled with tamoxifen-induced tdTomato were CD2+ monocytes in peripheral blood, 10% of dendritic cells were tdTomato+/CD2+ cells. Tamoxifen-independent expression of tdTomato occurred in approximately 3% of CD2+ macrophages, but in negligible (~0.5%) in CD2+ granulocytes.This work supplied a new transgenic mouse as a valuable tool for lineage tracing in CD2-expressing cells, for conditional mutant studies of immune modulatory effects in a time-dependent manner, and analysis of the potential therapeutic effect of CD2-targeting biologics.Copyright © 2025 Guo, Zhu, Yu, Li, Chen, Ci, Sun and Shen.
Adsorption properties of Merrifield-bCCA chelating resins: a new alternative for Pb2+ removal from water
García-Elías, Ochoa-Terán, López-Maldonado et al
RSC Adv (2025) 15 (12), 8999-9016
Abstract: In this work, five new chelating resins (MR n Bz) functionalized with N-benzyl bis(carbamoyl)carboxylic acid molecules (BzbCCA) on their surface were prepared to study the metal ion (M n+) adsorption properties in water. MR n Bz resins were characterized by FTIR, TGA, FESEM and EDS. The surface charge as a function of pH and the chemical adsorption of M n+ on the surface were evaluated through zeta potential (ζ) measurements. The M n+ adsorption capacity of MR n Bz resins was evaluated using Pb2+, Cu2+, Cd2+, and Ni2+ mixture model solutions at low concentrations. MR n Bz resins displayed selective adsorption of Pb2+ even in the presence of a molar excess of other cations, due to the intrinsic affinity and selectivity of BzbCCA molecules for this metal ion. The adsorption isotherms of Pb2+ showed that the adsorption capacity of MR n Bz resins was influenced by the spacer chain length. In addition, the resins were characterized by FTIR, TGA, FESEM and EDS after the M n+ adsorption process, confirming the M n+ loading on the resin surface.This journal is © The Royal Society of Chemistry.
Showing 1-4 of 19058 papers.
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CD2靶点信息
英文全称:T-cell surface antigen CD2
中文全称:T细胞表面抗原CD2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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