膜杰作
Star Staining
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The Human IL-1 R1 & IL-1 RAcP (Luc) HEK293 Reporter Cell was engineered to express NF-κB signaling response element. When stimulated with human IL-1 alpha or IL-1 beta protein, receptor-mediated signaling can drive NF-κB-mediated luminescence. Neutralization of biological effect of the ligand-receptor interaction by corresponding antibody results in a decrease in luminescence.
应用说明（Application）
• Screen for neutralizing antibodies blocking the stimulation of human IL-1 alpha or IL-1 beta protein.
• Screen for anti-human IL-1 R1 or IL-1 RAcP neutralizing antibody.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Complete Growth Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Application
Inhibition of human IL-1 alpha protein-induced reporter activity by anti-human IL-1 RAcP neutralization antibody.
The Human IL-1 R1 & IL-1 RAcP (Luc) HEK293 Reporter Cell was incubated with serial dilutions of antibodies in the presence of IL-1 alpha protein (Cat. No. ILA-H5214) with a final concentration of 0.02 μg/mL. The EC50 of anti-human IL-1 RAcP neutralization antibody is approximately 0.01347 μg/mL.
Protocol
Inhibition of human IL-1 beta protein-induced reporter activity by anti-human IL-1 RAcP neutralization antibody.
The Human IL-1 R1 & IL-1 RAcP (Luc) HEK293 Reporter Cell was incubated with serial dilutions of antibodies in the presence of IL-1 beta protein (Cat. No. ILB-H4110) with a final concentration of 0.0002 μg/mL. The EC50 of anti-human IL-1 RAcP neutralization antibody is approximately 0.01014μg/mL.
Protocol
Signaling Bioassay
Response to human IL-1 alpha protein (RLU).
The Human IL-1 R1 & IL-1 RAcP (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-1 alpha protein (Cat. No. ILA-H5214). The EC50 was approximately 0.1293 μg/mL.
Protocol
Response to human IL-1 alpha protein (FOLD).
The Human IL-1 R1 & IL-1 RAcP (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-1 alpha protein (Cat. No. ILA-H5214). The max induction fold was approximately 670.94.
Protocol
Response to human IL-1 beta protein (RLU).
The Human IL-1 R1 & IL-1 RAcP (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-1 beta protein (Cat. No. ILB-H4110). The EC50 was approximately 0.0007458 μg/mL.
Protocol
Response to human IL-1 beta protein (FOLD).
The Human IL-1 R1 & IL-1 RAcP (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-1 beta protein (Cat. No. ILB-H4110). The max induction fold was approximately 754.62.
Protocol
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Limited Use&License Disclosure
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE FOLLOWING TERMS OF LIMITED USE OF THIS CELL LINE PRODUCT.
- If the researcher is not willing to accept the terms of limited use of this cell line product, and the product is unused, ACRO will accept return of the unused product.
- Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for profit or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research.
- This cell line is neither intended for any animal or human therapeutic purposes nor for any direct human in vivo use . You have no right to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order.cn@acrobiosystems.com for further details.
