膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
P1F04
种属（Species）
Rabbit
亚型（Isotype）
Rabbit IgG | Rabbit Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Specifically recognizes Mouse IgG2b Fc,no recrecognizes Mouse IgG1 Fc,Mouse IgG2a Fc and Mouse IgG3 Fc.
免疫原（Immunogen）
Recombinant Mouse IgG2b Fc Protein is expressed from human 293T cells.
特异性（Specificity）
This product is a specific antibody specifically reacts with IgG2b.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-63 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
This product No cross-reactivity in ELISA with
Mouse IgG Fc, Tag Free (Cat. No. IG1-M5208).
Mouse IgG2a Fc, Tag Free (Cat. No. IGA-M5207).
Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214).
Mouse IgE Fc Protein, His Tag (Cat. No. IGE-M52H3).
Monoclonal Anti-Human Phospho-Tau (Thr217) Antibody, Mouse IgM (6G7C8) (Cat. No. PT7-Y2074).
Human IgG Fc, Tag Free (Cat. No. FCC-H5214).
Human IgG2 Fc, Tag Free (Cat. No. IG2-H5206).
Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200).
Human IgG4 Fc, Tag Free (Cat. No. IG4-H5205).
Mouse IgG3 Fc, Tag Free (Cat. No. IG3-M5201).
Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214).
电泳（SDS-PAGE）
Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) (Cat. No. IGB-MY2176) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mouse IgG2b Fc, Tag Free (Cat. No. IGB-M5203) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) (Cat. No. IGB-MY2176) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
背景（Background）
Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b.
