膜杰作
Star Staining
抗体来源（Source）
PE-Labeled Monoclonal Anti-His Antibody, Mouse IgG1 (55F8) is a mouse monoclonal antibody recombinantly expressed from Hybridoma.
克隆号（Clone）
55F8
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
偶联（Conjugate）
PE
抗体类型（Antibody Type）
Hybridoma Monoclonal
免疫原（Immunogen）
Purified his-tagged Protein.
特异性（Specificity）
Specifically recognizes the protein containing his Tag.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 3 months after reconstitution.
活性（Bioactivity）-FACS
Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with 100 μL of 10 μg/mL Human CD19 (20-291) Protein, His Tag (Cat. No. CD9-H52H2) and negative control protein respectively, washed and then followed with PE-Labeled Monoclonal Anti-His Antibody, Mouse IgG1 (55F8) (Cat. No. HIS-PFY63) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).
Protocol
背景（Background）
His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
