膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| RAS223-C01 | Pre-coated Anti-Influenza A (H1N1) Neuraminidase Antibody Microplate | 1 plate(8×12 strips) |
| RAS223-C02 | Influenza A [A/Victoria/4897/2022(H1N1)] Neuraminidase Standard | 15 μg |
| RAS223-C03 | HRP-Anti-Influenza A (H1N1) Neuraminidase Antibody | 20 μg |
| RAS223-C04 | 10×Washing Buffer | 50 mL |
| RAS223-C05 | Dilution Buffer | 50 mL |
| RAS223-C06 | Substrate Solution | 12 mL |
| RAS223-C07 | Stop Solution | 7 mL |
背景（Background）
Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.
应用说明（Application）
This kit is developed for Specific quantitative detection of Influenza A (H1N1) viruses Neuraminidase (NA) in samples.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Influenza A (H1N1) viruses Neuraminidase (NA). The kit consists of Pre-coated Anti-Influenza A (H1N1) Neuraminidase Antibody Microplate and Influenza A [A/Victoria/4897/2022(H1N1)] Neuraminidase Standard,HRP-Anti-Influenza A (H1N1) Neuraminidase Antibody and buffers.
Your experiment will include 5 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the Influenza A [A/Victoria/4897/2022(H1N1)] Neuraminidase as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add the HRP-Anti-Influenza A (H1N1) Neuraminidase Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
The kit has been tested to specifically identify Influenza A [A/Victoria/4897/2022(H1N1)] NA and Influenza A [Wisconsin/67/2022(H1N1)] NA, and do not identify Influenza A (A/Croatia/10136RV/2023) & (A/District of Columbia/27/2023) NA(H3N2), Influenza A [A/Massachusetts/18/2022(H3N2)] NA, Influenza A [A/Thailand/8/2022(H3N2)] NA, Influenza A [Darwin/6/2021] NA, Influenza A [A/Darwin/9/2021 (H3N2)] NA, Influenza B [Austria/1359417/2021] NA and Influenza B [PHUKET/3073/2013] NA.
