膜杰作
Star Staining
分子别名（Synonym）
IBP5,IGFBP5
表达区间及表达系统（Source）
Biotinylated Human IGFBP-5 Protein, Avitag,Flag&His Tag (IG5-H82D3) is expressed from human 293 cells (HEK293). It contains AA Leu 21 - Glu 272 (Accession # P24593).
Predicted N-terminus: Gly
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the N-terminus followed a Flag tag and at the C-terminus by a polyhistidine tag. The protein has a calculated MW of 33.3 kDa. The protein migrates as 38-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human IGFBP-5 Protein, Avitag,Flag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IGFBP-5 Protein, Avitag,Flag&His Tag (Cat. No. IG5-H82D3) is more than 90% and the molecular weight of this protein is around 35-55 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IGF-I Protein, Fc Tag (Cat. No. IG1-H5263) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGFBP-5 Protein, Avitag,Flag&His Tag (Cat. No. IG5-H82D3) with a linear range of 0.5-16 ng/mL (QC tested).
Protocol
背景（Background）
IGFBP5 stands for Insulin-like Growth Factor Binding Protein 5. It is a protein that belongs to the insulin-like growth factor binding protein (IGFBP) family. IGFBP5 plays a crucial role in regulating the activity and distribution of insulin-like growth factors (IGFs) in the body. Enables insulin-like growth factor I binding activity.
