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 >  Antibody>CD25 >FABm012-01

PE-Labeled Monoclonal Anti-Human CD25 Antibody, Mouse IgG1 (S561) (0.03% Proclin)

应用(Application)

Flow Cytometry (Evaluation of the expression of CD25 on Human cells).

克隆号(Clone)

S561

种属(Species)

Mouse

亚型(Isotype)

Mouse IgG1 | Mouse Kappa

特异性(Specificity)

This product is a specific antibody specifically reacts with CD25 protein

种属反应性(Reactivity)

Human

免疫原(Immunogen)

Purified Human CD25 Protein.

偶联(Conjugate)

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

同型对照(Isotype Control)

The Isotype control is sold separately and you can search for Cat. No. DNP-PM1 for product information.

推荐稀释比(Recommended Dilution)

1:20

制剂(Formulation)

Supplied as 0.2 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin300 with trehalose as protectant.

Contact us for customized product form or formulation.

存储(Storage)

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. Store at 2-8 °C for 12 months.
 

活性(Bioactivity)-FACS

CD25 FACS

Flow cytometric analysis of Activated Human PBMC cells staining with PE-Labeled Monoclonal Anti-human CD25 Antibody, Mouse IgG1 (S561)(Cat. No. FABm012-01) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

IL-2 R alpha, also known as CD25, is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

 

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