应用(Application)
Flow Cytometry (Evaluation of the expression of CD25 on Human cells).
克隆号(Clone)
S561
种属(Species)
Mouse
亚型(Isotype)
Mouse IgG1 | Mouse Kappa
特异性(Specificity)
This product is a specific antibody specifically reacts with CD25 protein
种属反应性(Reactivity)
Human
免疫原(Immunogen)
Purified Human CD25 Protein.
偶联(Conjugate)
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
同型对照(Isotype Control)
The Isotype control is sold separately and you can search for Cat. No. DNP-PM1 for product information.
推荐稀释比(Recommended Dilution)
1:20
制剂(Formulation)
Supplied as 0.2 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin300 with trehalose as protectant.
Contact us for customized product form or formulation.
存储(Storage)
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- Store at 2-8 °C for 12 months.
活性(Bioactivity)-FACS
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Flow cytometric analysis of Activated Human PBMC cells staining with PE-Labeled Monoclonal Anti-human CD25 Antibody, Mouse IgG1 (S561)(Cat. No. FABm012-01) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).
Protocol
背景(Background)
IL-2 R alpha, also known as CD25, is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.