膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (M1B12) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
M1B12
种属（Species）
Rabbit
亚型（Isotype）
Rabbit IgG | Rabbit Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
免疫原（Immunogen）
MMAF-BSA.
特异性（Specificity）
Specifically recognizes MMAF, no recrecognizes MMAE.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.06-500 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
This product No cross-reactivity in ELISA with
Disitamab Vedotin (RC48).
Trastuzumab Deruxtecan.
Trastuzumab-DM1.
Trastuzumab Biosimilar.
电泳（SDS-PAGE）
Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (M1B12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (M1B12) (Cat. No. MMF-MY2220) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (M1B12) (Cat. No. MMF-MY2220) at 1 μg/mL (100 μL/well) can bind IgG1-MMAF with a linear range of 0.06-31 ng/mL (QC tested).
Protocol
Serial dilutions of MMAF were added into Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (M1B12) (Cat. No. MMF-MY2220): IgG1-MMAF binding reactions. The half maximal inhibitory concentration (IC50) is 0.6853 μg/mL (Routinely tested).
Protocol
背景（Background）
Monomethyl auristatin F (MMAF) is a potent mitotic inhibitor that inhibits tubulin polymerization. MMAF is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several cancer types. Anti-MMAF antibody is a rabbit monoclonal antibody specifically reacts with MMAF without MMAE, which is more sensitive than mouse antibody. The anti-MMAF antibody is a useful reagent in PK assay to determine conjugated antibodies.
