膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
1M2B1
种属（Species）
Rabbit
亚型（Isotype）
Rabbit IgG | Rabbit Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
免疫原（Immunogen）
Doxorubicin-BSA.
特异性（Specificity）
Specifically recognizes Doxorubicin.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.06-125 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
This product can cross in Elisa with
Doxorubicin.
This product No cross-reactivity in ELISA with
(PBD)SG3199.
MMAF.
Monomethyl auristatin E.
Trastuzumab Biosimilar.
电泳（SDS-PAGE）
Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) (Cat. No. DON-MY2215) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Doxorubicin-ADC at 0.1 μg/mL (100 μL/well) can bind Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) (Cat. No. DON-MY2215) with a linear range of 0.06-8 ng/mL (QC tested).
Protocol
背景（Background）
Doxorubicin a cytotoxic anthracycline antibiotic, is an anti-cancer chemotherapy agent. As the payload of ADC drugs, Doxorubicin can act directly on DNA, insert into the double helix strands of DNA, cause the latter to unwind, change the template properties of DNA, and inhibit DNA polymerase, thereby inhibiting the synthesis of both DNA and RNA. Anti-Doxorubicin antibody is a rabbit monoclonal antibody specifically reacts with Doxorubin without other payloads, which is more sensitive than mouse antibody. The anti-Doxorubicin antibody is a useful reagent in PK assay to determine conjugated antibodies.
