膜杰作
Star Staining
分子别名（Synonym）
NECTIN4,LNIR, PRR4, PVRL4
表达区间及表达系统（Source）
PE-Labeled Human Nectin-4 Protein, His Tag (NE4-HP2H4) is produced via site-specific conjugation of PE to Human Nectin-4 Protein, His Tag under optimal conditions with a proprietary technology. Human Nectin-4 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1).
Predicted N-terminus: Gly 32
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 37.8 kDa.
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-FACS
5e5 of Anti-Nectin-4 CAR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human Nectin-4 Protein, His Tag (Cat. No. NE4-HP2H4) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).
Protocol
背景（Background）
Nectin-4(Nectin cell adhesion molecule 4) is a Nectin and nectin-like family of cell adhesion molecules. It is found in high levels in normal embryonic and fetal tissues, but decreases after adulthood and is limited in distribution in healthy tissues. Nectin-4 is overexpressed in a variety of tumor cells and promotes tumor cell growth and proliferation. Solid tumors with high expression of Nectin-4 include bladder cancer, pancreatic cancer, triple negative breast cancer, non-small cell lung cancer, stomach cancer, esophageal cancer, ovarian cancer and many other cancers. Studies have found that Nectin-4 promotes tumor cell proliferation, differentiation, migration and invasion by activating the PI3K/Akt pathway, and is believed to play an important role in the occurrence and metastasis of cancer. It is based on these research backgrounds that drug research targeting this target has emerged.
