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 >  Protein>DLL1 >DL1-H52H8

Human DLL1 / Delta1 Protein, His Tag, premium grade

分子别名(Synonym)

DLL1,Delta1,H-Delta-1

表达区间及表达系统(Source)

Human DLL1 Protein, His Tag, premium grade (DL1-H52H8) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Gly 540 (Accession # NP_005609).

Predicted N-terminus: Gln 18

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Request for sequence

蛋白结构(Molecular Characterization)

DLL1 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 58.4 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

无菌(Sterility)

Negative

支原体(Mycoplasma)

Negative.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

DLL1 SDS-PAGE

Human DLL1 Protein, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

DLL1 SEC-MALS

The purity of Human DLL1 Protein, His Tag, premium grade (Cat. No. DL1-H52H8) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-BLI

DLL1 BLI

Loaded Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) on Protein A Biosensor, can bind Human DLL1 Protein, His Tag, premium grade (Cat. No. DL1-H52H8) with an affinity constant of 290 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Delta-like protein 1 (DLL1) is also known as Drosophila Delta homolog 1 (Delta1 or H-Delta-1), which contains one DSL domain and eight EGF-like domains. DLL1 is ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. As for expression, DLL1 is expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. Furthermore, DLL1 acts as a ligand for Notch receptors. Also, DLL1 can block the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

 

前沿进展

 
 
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DLL1靶点信息
英文全称:Delta Like Canonical Notch Ligand 1
中文全称:Delta样蛋白1
种类:
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:临床前
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