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 >  Protein>Fc gamma RIIIA / CD16a >CD8-H52H4

Human Fc gamma RIIIA / CD16a (V176) Protein, His Tag (SPR & BLI & MALS verified)

分子别名(Synonym)

FCGR3A,CD16A,FCG3,FCGR3,IGFR3

表达区间及表达系统(Source)

Human CD16a (V176), His Tag (CD8-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1 (F176V)).

Predicted N-terminus: Gly 17

Request for sequence

蛋白结构(Molecular Characterization)

Fc gamma RIIIA / CD16a Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Fc gamma RIIIA / CD16a SDS-PAGE

Human CD16a (V176), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Fc gamma RIIIA / CD16a SEC-MALS

The purity of Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) is more than 95% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-SPR

Fc gamma RIIIA / CD16a SPR

Immobilized Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) on CM5 Chip via anti-His antibody, can bind  Herceptin with an affinity constant of 163 nM as determined in SPR assay (Biacore T200) (QC tested).

Protocol

Fc gamma RIIIA / CD16a BATCH SPR
Fc gamma RIIIA / CD16a SPR

Herceptin captured on Protein A Chip can bind Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) with an affinity constant of 0.124 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

Protocol

Fc gamma RIIIA / CD16a SPR

Herceptin immobilized on CM5 Chip can bind Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) with an affinity constant of 0.215 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Fc gamma RIIIA / CD16a BLI

Loaded Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 0.447 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Fc gamma RIIIA / CD16a BLI

Loaded Herceptin on Protein A Biosensor, can bind Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) with an affinity constant of 0.37 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Fc gamma RIIIA / CD16a BLI

Loaded Herceptin on FAB2G Biosensor, can bind Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) with an affinity constant of 0.31 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages.
CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

文献引用(Citations)

 

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Fc gamma RIIIA / CD16a靶点信息
英文全称:Low affinity immunoglobulin gamma Fc region receptor III-A
中文全称:低亲和力免疫球蛋白γFc段受体III-A
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:12详情
最高研发阶段:临床二期
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