登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>GFR alpha-1 >GF1-H5224

Human GFR alpha-1 Protein, His Tag

热销产品推荐:

ACROBiosystems提供针对多种生物标志物靶标的lHC抗体产品,具有:验证充分、质量可靠、灵活授权及免疫组化检测服务支持!

分子别名(Synonym)

GFRA1,GDNFRA,RETL1,TRNR1,GDNFR,GFR-ALPHA-1,RET1L

表达区间及表达系统(Source)

Human GFR alpha-1, His Tag (GF1-H5224) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Ser 424 (Accession # NP_665736.1).

Predicted N-terminus: Asp 25

Request for sequence

蛋白结构(Molecular Characterization)

GFR alpha-1 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 46.6 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

GFR alpha-1 SDS-PAGE

Human GFR alpha-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

GFR alpha-1 ELISA

Immobilized Human GDNF Protein, premium grade (Cat. No. GDF-H5219) at 2 μg/mL (100 μL/well) can bind Human GFR alpha-1 Protein, His Tag (Cat. No. GF1-H5224) with a linear range of 0.5-2 ng/mL (Routinely tested).

Protocol

 

背景(Background)

GDNF family receptor alpha-1 (GFRA1) is also known as RET ligand 1 (RETL1), TGF-beta-related neurotrophic factor receptor 1 (TRNR1), is a novel glycosylphosphatidylinositol (GPI)-linked cell surface receptor, and belongs to the glial cell line-derived neurotrophic factor (GDNF) receptor subfamily. The GDNF family ligands comprises GDNF, Neurturin (NTN), Artemin, and Persephin. GDNF and NTN are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GFRA1 mediates the association with and activation of the RET tyrosine kinase receptor (RTK), and subsequently initiates the RET signaling pathway. GFR alpha-mediated signaling plays an important role in the survival, differentiation, and migration of central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system, as well as the inflammatory response in some carciniomas.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
GFR alpha-1靶点信息
英文全称:Glial cell line-derived neurotrophic factor receptors
中文全称:神经胶质细胞系衍生神经营养因子受体
种类:
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定