膜杰作
Star Staining
分子别名（Synonym）
MET,AUTS9,HGFR,RCCP2,c-Met
表达区间及表达系统（Source）
Human HGF R, His Tag (MET-H5227) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932 (Accession # AAI30421.1).
Predicted N-terminus: Glu 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus. The mature form of HGFR is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.5 kDa and 70.2 kDa. The protein migrates as 40-45 kDa and 85-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
Human HGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human HGF R, His Tag (Cat. No. MET-H5227) is more than 85% and the molecular weight of this protein is around 100-140 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human HGF/Hepatocyte Growth Factor at 2 μg/mL (100 μL/well) can bind Human HGF R, His Tag (Cat. No. MET-H5227) with a linear range of 0.039-0.313 μg/mL (Routinely tested).
Protocol
+添加评论
- 182XXXXXXX4
- This protein has a particularly good affinity and is relatively stable. It is dissolved under recommended conditions, and there is little difference between batches when used. In ELISA experiments, it has good reproducibility whether used as a coating or as a standard substance. protocal is very detailed, there are giveaways, logistics is fast
- 2023-4-12
- 651XXXXXXX
- The c-MET protein was delivered on time and we were able to use this protein for ELISA screening. I was able to get really good results from using this protein and would come back to Acrobiosystems to reorder this protein or order other proteins that I will need for future experiments.
- 2022-6-10
- 188XXXXXXX9
- 此蛋白亲和力特别好，而且比较稳定，在推荐的条件下进行溶解处理，使用时不同批次之间差异很小，在ELISA实验中，不管是用于包被还是标准品都有很好的重现性，后续还会继续使用，而且也会尝试其他蛋白。protocal也很详细，还有赠品，物流也快
- 2023-3-19
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背景（Background）
Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.
