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 >  Protein>IL-1RL1 >IL1-H5250

Human IL-1RL1 / ST2 Protein, Fc Tag

分子别名(Synonym)

IL1RL1,DER4,FIT-1,IL33R,ST2,ST2L,ST2V,T1

表达区间及表达系统(Source)

Human IL-1RL1, Fc Tag (IL1-H5250) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Pro 323 (Accession # NP_003847).

Predicted N-terminus: Lys 19

Request for sequence

蛋白结构(Molecular Characterization)

IL-1RL1 Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 61.0 kDa. The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-1RL1 SDS-PAGE

Human IL-1RL1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

IL-1RL1 ELISA

Immobilized Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) at 5 μg/mL (100 μL/well) can bind Human IL-33 Protein, His Tag (Cat. No. IL3-H52H7) with a linear range of 5-40 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-BLI

IL-1RL1 BLI

Loaded Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) on Protein A Biosensor, can bind with Biotinylated Human IL-33, His,Avitag (Cat. No. IL3-H82H5) an affinity constant of 1.81 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin 1 receptor-like 1 (IL1RL1) is also known as ST2, DER4, FIT-1, IL33R, ST2L, ST2V, which belongs to the interleukin-1 receptor family. IL1RL1 contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RL1 is receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL1RL1 possibly involved in helper T-cell function. IL1RL1 can interact with MYD88, IRAK1, IRAK4, and TRAF6.

 

前沿进展

 
 
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IL-1RL1靶点信息
英文全称:Interleukin-1 receptor-like 1
中文全称:白介素1受体样蛋白1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:5详情
最高研发阶段:申请上市
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