膜杰作
Star Staining
分子别名（Synonym）
IL1RL1,DER4,FIT-1,IL33R,ST2,ST2L,ST2V,T1
表达区间及表达系统（Source）
Human IL-1RL1, Fc Tag (IL1-H5250) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Pro 323 (Accession # NP_003847).
Predicted N-terminus: Lys 19
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 61.0 kDa. The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
Human IL-1RL1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) at 5 μg/mL (100 μL/well) can bind Human IL-33 Protein, His Tag (Cat. No. IL3-H52H7) with a linear range of 5-40 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) on Protein A Biosensor, can bind with Biotinylated Human IL-33, His,Avitag (Cat. No. IL3-H82H5) an affinity constant of 1.81 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
+添加评论
- 181XXXXXXX6
- 我们购买这款蛋白用于筛选抗体，主要用在ELISA实验，这款蛋白灵敏度高，蛋白活性很好，实验取得了很好的数据，一直在Acro购买蛋白，Acro值得信赖。
- 2023-8-30
- 166XXXXXXX8
- 我们应用于于抗体筛选，经常购买贵公司的acro的产品，包括Elisa，总之贵公司的产品很不错，是筛选与Elisa的首选蛋白，还会经常回购，涉及公司保密问题，图就不放了。
- 2022-11-4
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背景（Background）
Interleukin 1 receptor-like 1 (IL1RL1) is also known as ST2, DER4, FIT-1, IL33R, ST2L, ST2V, which belongs to the interleukin-1 receptor family. IL1RL1 contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RL1 is receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL1RL1 possibly involved in helper T-cell function. IL1RL1 can interact with MYD88, IRAK1, IRAK4, and TRAF6.
