膜杰作
Star Staining
分子别名（Synonym）
CD113,Nectin-3,PVRL3
表达区间及表达系统（Source）
Human Nectin-3, Fc Tag (PV3-H5255) is expressed from human 293 cells (HEK293). It contains AA Gly 58 - Asp 400 (Accession # Q9NQS3).
Predicted N-terminus: Gly 58
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 64.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Nectin-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human Nectin-1, His Tag (Cat. No. PV1-H5223) at 2 μg/mL (100 μL/well) can bind Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) with a linear range of 5-78 ng/mL (QC tested).
Protocol
Immobilized Human TIGIT, His Tag (Cat. No. TIT-H52H5) at 1 μg/mL (100 μL/well) can bind Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) with a linear range of 0.078-5 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.732 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) on Protein A Biosensor, can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with an affinity constant of 0.52 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human Nectin-1, His Tag (Cat. No. PV1-H5223) on HIS1K Biosensor, can bind Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) with an affinity constant of 0.14 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Nectins are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin system in epithelial cells. PVRL3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Furthermore, PVRL3 induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.
