登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>PLAU >PLU-H5229

Human PLAU / uPA Protein, His Tag

分子别名(Synonym)

Urokinase,PLAU,ATF,UPA,URK,u-PA,BDPLT5,QPD

表达区间及表达系统(Source)

Human PLAU, His Tag (PLU-H5229) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431 (Accession # NP_002649.1).

Predicted N-terminus: Ser 21

Request for sequence

蛋白结构(Molecular Characterization)

PLAU Structure

Human PLAU, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 47.2 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Please note that the protein is not activated by protease digestion. In vitro activation is generally recommended for higher activity.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>92% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Sodium Acetate, 100 mM NaCl, pH5.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

PLAU SDS-PAGE

Human PLAU, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 92%.

 

活性(Bioactivity)-ELISA

PLAU ELISA

Immobilized Human PLAU, His Tag (Cat. No. PLU-H5229) at 5 μg/mL (100 μL/well) can bind Biotinylated Human uPAR, His,Avitag (Cat. No. UPR-H82E7) with a linear range of 0.3-5 ng/mL (QC tested).

Protocol

 

背景(Background)

Urokinase - type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys – Pro – Gly - Arg560 - Val561 – Val – Gly – Gly – Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).

文献引用(Citations)

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
PLAU靶点信息
英文全称:Urokinase plasminogen activator
中文全称:尿激酶型纤维蛋白溶酶原激活因子
种类:Homo sapiens
上市药物数量:4详情
临床药物数量:6详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定