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 >  Protein>Reg4 >RE4-H5221

Human Reg4 Protein, His Tag

分子别名(Synonym)

REG4,GISP,REG-IV,RELP

表达区间及表达系统(Source)

Human Reg4, His Tag (RE4-H5221) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Pro 158 (Accession # AAH17089).

Predicted N-terminus: Asp 23

Request for sequence

蛋白结构(Molecular Characterization)

Reg4 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 16.7 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Reg4 SDS-PAGE

Human Reg4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

背景(Background)

Regenerating islet-derived protein 4 (REG4) is also known as Gastrointestinal secretory protein (GISP), REG-like protein (RELP). REG4 contains one C-type lectin domain. REG4 has calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. REG4 may be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.

 

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