膜杰作
Star Staining
分子别名（Synonym）
ROR2,NTRKR2
表达区间及表达系统（Source）
Human ROR2, Fc Tag (RO2-H5251) is expressed from human 293 cells (HEK293). It contains AA Glu 34 - Gly 403 (Accession # A1L4F5-1).
Predicted N-terminus: Glu 34
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 68 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human ROR2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human ROR2, Fc Tag (Cat. No. RO2-H5251) at 1 μg/mL (100 μL/well) can bind Anti-ROR2 Antibody, Human IgG1 with a linear range of 2-8 ng/mL (QC tested).
Protocol
背景（Background）
Tyrosine-protein kinase transmembrane receptor ROR2 is also known as Neurotrophic tyrosine kinase, receptor-related 2 (NTRKR2), which belongs to the protein kinase superfamily and Tyr protein kinase family and ROR subfamily. ROR2 is a homodimer protein, which can binds YWHAB or interact with WTIP. ROR2 may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development.
