膜杰作
Star Staining
分子别名（Synonym）
SERPINH1,Serpin H1,HSP47,CBP1,CBP2,SERPINH2,PIG14,AsTP3,Colligin,OI10,PPROM,RA-A47,gp46
表达区间及表达系统（Source）
Human Serpin H1, His Tag (SE1-H5224) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Leu 418 (Accession # P50454-1).
Predicted N-terminus: Ala 19
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 45.5 kDa. The protein migrates as 48-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Serpin H1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
背景（Background）
Serpin H1 is also known as SERPINH1, 47 kDa heat shock protein (HSP47), Collagen-binding protein (Colligin), Arsenic-transactivated protein 3 (AsTP3), which belongs to the serpin family. SERPINH1 binds specifically to collagen. SERPINH1 / HSP47 could be involved as a chaperone in the biosynthetic pathway of collagen.
