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 >  Protein>SIRP gamma >SIG-H5253

Human SIRP gamma / CD172g Protein, Fc Tag

分子别名(Synonym)

SIRPG,CD172g,SIRPB2,SIRP-gamma,SIRP-b2,SIRP-beta-2,Cripto-1

表达区间及表达系统(Source)

Human SIRP gamma, Fc Tag (SIG-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 29 - Ser 364 (Accession # AAH64532).

Predicted N-terminus: Glu 29

Request for sequence

蛋白结构(Molecular Characterization)

SIRP gamma Structure

This protein carries a human IgG1 Fc tag at the C-terminus

The protein has a calculated MW of 63.7 kDa. The protein migrates as 67-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

SIRP gamma SDS-PAGE

Human SIRP gamma, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

SIRP gamma ELISA

Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 5 μg/mL (100 μL/well) can bind Human SIRP gamma, Fc Tag (Cat. No. SIG-H5253) with a linear range of 20-312 ng/mL (QC tested).

Protocol

 

背景(Background)

Signal-regulatory protein gamma (SIRPG or SIRP-gamma) is also known as CD172 antigen-like family member B (CD172g), Signal-regulatory protein beta-2 (SIRPB2), is a single-pass type I membrane protein. SIRPG is expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. SIRPG interacts with CD47. On binding with CD47, CD172g mediates cell-cell adhesion.

 

前沿进展

 
 
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SIRP gamma靶点信息
英文全称:Teratocarcinoma-derived growth factor 1
中文全称:畸胎瘤衍生生长因子-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:无进展
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