膜杰作
Star Staining
分子别名（Synonym）
TIGIT,VSIG9,VSTM3
表达区间及表达系统（Source）
Rabbit TIGIT, Fc Tag (TIT-R5259) is expressed from human 293 cells (HEK293). It contains AA Ala 16 - Pro 142 (Accession # G1SLC1).
Predicted N-terminus: Ala 16
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 40.4 kDa. The protein migrates as 45-50 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Rabbit TIGIT, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rabbit TIGIT, Fc Tag (Cat. No. TIT-R5259) is more than 90% and the molecular weight of this protein is around 90-120 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Nectin-2, His Tag (Cat. No. PV2-H52E2) at 5 μg/mL (100 μL/well) can bind Rabbit TIGIT, Fc Tag (Cat. No. TIT-R5259) with a linear range of 5-78 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Rabbit TIGIT, Fc Tag (Cat. No. TIT-R5259) on Protein A Biosensor, can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with an affinity constant of 0.21 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
