登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>IL-17 RA >ILA-H82F1

Biotinylated Human IL-17 RA / CD217 Protein, Fc,Avitag™

分子别名(Synonym)

CD217,CDw217,IL-17RA,IL17R,CANDF5,hIL-17R

表达区间及表达系统(Source)

Biotinylated Human IL-17 RA, Fc,Avitag (ILA-H82F1) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Trp 320 (Accession # Q96F46-1).

Predicted N-terminus: Leu 33

Request for sequence

蛋白结构(Molecular Characterization)

IL-17 RA Structure

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 61.9 kDa. The protein migrates as 80-115 kDa under reducing (R) condition, and 150-200 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-17 RA SDS-PAGE

Biotinylated Human IL-17 RA, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

IL-17 RA ELISA

Immobilized Human IL17A Protein, premium grade (Cat. No. ILA-H5219) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) with a linear range of 2-125 ng/mL (QC tested).

Protocol

IL-17 RA ELISA

Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) with a linear range of 0.02-0.313 μg/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

IL-17 RA BLI

Loaded Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) on SA Biosensor, can bind Human IL17A, Tag Free with an affinity constant of 1.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin 17 receptor A (IL17RA) is also known as cluster of differentiation w217 (CDw217), is a pro-inflammatory cytokine secreted by activated T-lymphocytes, belong to ubiquitous type I membrane glycoprotein, and binds with low affinity to interleukin 17A (IL17A). IL 17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested . IL 17RA associates with IL 17RC to form a signaling receptor complex for IL 17 and IL 17F . Ligand and IL 17RA ligation promotes T cell activation and the production of IL - 6, G-CSF, SCF, and multiple pro-inflammatory chemokines. Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5).

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
IL-17 RA靶点信息
英文全称:Interleukin-17 receptor A
中文全称:白细胞介素-17受体A亚基
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:1详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定