膜杰作
Star Staining
分子别名（Synonym）
GREM1,CKTSF1B1,DAND2,DRM,PIG2
表达区间及表达系统（Source）
Human Gremlin, His Tag (GR1-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 25 - Asp 184 (Accession # NP_037504).
Predicted N-terminus: Lys 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.2 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 10 mM Sodium Citrate, pH3.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Gremlin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human BMP-2, Tag Free (Cat. No. BM2-H4117) at 2 μg/mL (100 μL/well) can bind Human Gremlin, His Tag (Cat. No. GR1-H52H3) with a linear range of 0.02-0.313 μg/mL (QC tested).
Protocol
背景（Background）
Gremlin is also known as Cysteine knot superfamily 1, BMP antagonist 1 (CKTSF1B1), DAN domain family member 2 (DAND2), Down-regulated in Mos-transformed cells protein (DRM), Increased in high glucose protein 2 (IHG-2), Cell proliferation-inducing gene 2 protein (PIG2) or Gremlin-1 (GREM1), which is highly expressed in small intestine, fetal brain and colon. Gremlin / GREM-1 interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. Gremlin may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Gremlin down-regulates the BMP4 signaling in a dose-dependent manner and acts as inhibitor of monocyte chemotaxis.
