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 >  Protein>IFN-gamma >IFG-H4211

Human IFN-gamma / IFNG Protein, premium grade

GMP version GMP-IFGH24 is now available for seamless transition.

分子别名(Synonym)

Interferon-gamma ,Interferon-γ

表达区间及表达系统(Source)

Human IFN-gamma, premium grade (IFG-H4211) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Gln 166 (Accession # P01579).

Predicted N-terminus: Gln 24

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Request for sequence

蛋白结构(Molecular Characterization)

IFN-gamma Structure

This protein carries no "tag".

The protein has a calculated MW of 16.8 kDa. The protein migrates as 15 kDa, 19 kDa and 24 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

宿主蛋白残留(Host Cell Protein)

<0.5 ng/µg of protein tested by ELISA.

宿主核酸残留(Host Cell DNA)

<0.02 ng/μg of protein tested by qPCR.

无菌(Sterility)

Negative

支原体(Mycoplasma)

Negative.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IFN-gamma SDS-PAGE

Human IFN-gamma, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

IFN-gamma SEC-MALS

The purity of Human IFN-gamma, premium grade (Cat. No. IFG-H4211) is more than 90% and the molecular weight of this protein is around 34-44 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-Bioactivity CELL BASE

IFN-gamma CELL

Human IFN-gamma, premium grade (Cat. No. IFG-H4211) inhibits the proliferation of HT-29 cells. The specific activity of Human IFN-gamma, premium grade is > 2.00×10^7 IU/mg, which is calibrated against human interferon gamma Standard (NIBSC code: 87/586) (QC tested).

Protocol

 

活性(Bioactivity)-ELISA

IFN-gamma ELISA

Immobilized Human IFN-gamma, premium grade (Cat. No. IFG-H4211) at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, Fc Tag (Cat. No. IF1-H5254) with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

 

稳定性(Stability)

IFN-gamma STABILITY

The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IFN-gamma.

 

背景(Background)

Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.

文献引用(Citations)

 

前沿进展

 
 
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IFN-gamma靶点信息
英文全称:Interferon gamma
中文全称:干扰素-γ
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:9详情
最高研发阶段:批准上市
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