Amyloid beta 40分子别名
Amyloid beta 40
Amyloid beta 40分子背景
Amyloid beta 40 ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Amyloid beta 40 in cerebrospinal fluid, serum, plasma or other biological fluids. An antibody specific for Amyloid beta 40 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Amyloid beta 40 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Amyloid beta 40 Antibody, which also binds the Amyloid beta 40 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Amyloid beta 40 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Amyloid beta 40 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Amyloid beta 40 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Amyloid beta 40 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.