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新冠疫苗开发与评价的核心试剂

背景
由新冠病毒(SARS-CoV-2)引起的COVID-19大流行是一个世纪以来人类面临的最严峻挑战,一种安全有效的疫苗对于控制疫情和防止再次暴发具有重要意义。目前,针对COVID-19的疫苗研发进展飞快,病毒灭活疫苗、基因工程重组疫苗、病毒载体类疫苗、核酸类疫苗(质粒DNA、mRNA等)等多条技术路线齐头并进,其中已经有多款新冠候选疫苗进入3期临床试验阶段,其安全性、免疫原性、有效性等问题备受关注。
ACROBiosystems基于在重组蛋白领域多年的深耕和技术积累,针对新冠病毒疫苗研究领域开发了包括经MALS和负染电镜验证的三聚体S蛋白在内的多种重组蛋白、检测试剂盒等核心试剂,可用于检测IgG/M抗体滴度、中和抗体滴度、抗原滴度等,助力新冠病毒疫苗开发与评价。
应用与产品
应用场景1:IgG抗体滴度检测
> Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike protein RBD)
S RBD蛋白抗体滴度检测试剂盒采用间接ELISA法检测血清中新冠病毒抗体IgG滴度,检测灵敏度为24 ng/mL
Immobilized SARS-CoV-2 S protein RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG antibody with sensitivity of 24 ng/mL
> SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)
蛋白纯度、分子量和聚体形式经SDS-PAGE, SEC-MALS 和负染电镜技术严格验证

The purity of SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) was more than 90% verified by SDS-PAGE under reducing (R) condition. The molecular weight was around 550-660 kDa confirmed by SEC-MALS. The particles are similar in size and appearance to SARS-CoV-2 trimers reported in published literature verified by negative stain electron micrography.

Protocol

ELISA验证S蛋白与新冠病毒抗体结合活性强

Immobilized Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) with a linear range of 0.3-10 ng/mL

Protocol

> Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)
ELISA验证S RBD蛋白与新冠病毒抗体结合活性强
Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL
应用案例:
辉瑞和BioNTech新冠疫苗1/2期临床研究在检测与RBD结合的IgG抗体水平时,使用了ACROBiosystems生产的生物素化RBD蛋白(货号:SPD-C82E9),通过将此蛋白与链霉亲和素包被的微粒结合,来捕获血液样本中结合RBD的IgG抗体,获得了满意的实验结果。[1]

Phase 1/2 Study to Describe the Safety and Immunogenicity of a COVID-19 RNA Vaccine Candidate (BNT162b1) in Adults 18 to 55 Years of Age: Interim Report. medRxiv preprint. doi: https://www.medrxiv.org/content/10.1101/2020.06.30.20142570v1

应用场景2:中和抗体滴度检测
> Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit
中和抗体滴度检测试剂盒可检测血清中的中和抗体
Inhibition of Human ACE2: SARS-CoV-2 Spike RBD (HRP) binding by Anti-SARS-CoV-2 Neutralizing Antibody measured by competitive ELISA Assay.
Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody (Cat. No. TAS022-C02) (1:2 serial dilutions, from 1.25 μg/mL to 0.0012 μg/mL) was added into Human ACE2: SARS-CoV-2 Spike RBD (HRP) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting. A representative result showed that the IC50 was calculated to be 0.06 μg/mL
> SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)
ELISA验证,S RBD蛋白与RBD中和抗体结合活性强

Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.1-3 ng/mL

Protocol

ELISA验证,S RBD蛋白与ACE2蛋白结合活性强

Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-3 ng/mL

Protocol

应用场景3:抗原滴度检测
> SARS-CoV-2 Spike Protein Titer Assay Kit
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike protein. The kit consists of pre-coated Anti-SARS-CoV-2 Spike Protein Antibody Microplate, SARS-CoV-2 Spike protein as Control, biotin-Anti-SARS-CoV-2 Spike protein antibody and HRP-Streptavidin. Detection is performed using HRP-Streptavidin with sensitivity of 200 pg/mL.
应用场景4:抗体亚型检测

ACROBiosystems还开发了抗体亚型检测系列产品,可用于新冠病毒抗体IgG1、IgG2、IgG3和IgG4四种抗体亚型的检测。

> Anti-SARS-CoV-2 IgG1/ IgG 2/ IgG 3/ IgG 4 Titer Serologic Assay Kit (S Protein RBD)

Fig.4 抗体亚型检测交叉验证数据图

相关文章阅读:新冠疫苗捷报频传,但ADE效应你清楚了吗?

产品列表
重组蛋白类
分子 货号 产品名称
ACE2 protein AC2-H52H8 Human ACE2 / ACEH Protein, His Tag (MALS verified)
AC2-H82E6 Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified)
AC2-H5257 Human ACE2 / ACEH Protein, Fc Tag (MALS verified)
AC2-H82F9 Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™
S trimer protein SPN-C52H9 SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)
SPN-C82E9 Biotinylated SARS-CoV-2 S protein, His,Avitag™, Super stable trimer (MALS verified)
SPN-C52H3 SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified)
SPN-C82E3 Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag™, Super stable trimer (MALS verified)
S protein RBD SPD-C52H3 SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)
SPD-C82E9 Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)
SPD-C5259 SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag(MALS verified)
SPD-C82Aa Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified)
S1 protein S1N-C52H4 SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified)
S1N-C82E8 Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified)
S1N-C5256 SARS-CoV-2 (COVID-19) S1 protein (D614G), His Tag
S1N-C82E3 Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His,Avitag™
S1 protein NTD S1D-C52H6 SARS-CoV-2 (COVID-19) S1 protein NTD, His Tag
S1 protein CTD S1D-C52H3 SARS-CoV-2 (COVID-19) S1 protein CTD, His Tag (MALS-verified)
S2 protein S2N-C52H5 SARS-CoV-2 (COVID-19) S2 protein, His Tag
Nucleocapsid protein NUN-C5227 SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag
NUN-C82E8 Biotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His,Avitag™
试剂盒类
类别 分子 货号 产品描述 订购/预购
中和抗体滴度检测试剂盒Spike RBD TAS-K022 Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit

订购

Spike RBD RAS-N028 Anti-SARS-CoV-2 (B.1.1.7) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)

点击预购

Spike RBD RAS-N031 Anti-SARS-CoV-2 (B.1.351) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)

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Spike RBD RAS-N034 Anti-SARS-CoV-2 (P.1) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)

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抗体滴度检测试剂盒 Spike S1 RAS-T001 Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike S1)

订购

Spike RBD TAS-K002 Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike RBD)

订购

Spike protein TAS-K007 Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike Protein)

订购

Nucleocapsid Protein RAS-T008 Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Nucleocapsid Protein)

订购

Spike RBD RAS-T009 Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD)

订购

Spike S1 RAS-T012 Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay kit (Spike S1)

订购

Spike RBD RAS-T013 Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay kit (Spike RBD)

订购

Spike RBD RAS-T018 Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit

订购

Spike RBD RAS-T019 Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit

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Spike RBD RAS-T026 Anti-SARS-CoV-2 (B.1.1.7) Antibody IgG Titer Serologic Assay kit (Spike RBD)

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Spike protein RAS-T027 Anti-SARS-CoV-2 (B.1.1.7) Antibody IgG Titer Serologic Assay kit (Spike Protein)

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Spike RBD RAS-T029 Anti-SARS-CoV-2 (B.1.351) Antibody IgG Titer Serologic Assay kit (Spike RBD)

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Spike protein RAS-T030 Anti-SARS-CoV-2 (B.1.351) Antibody IgG Titer Serologic Assay kit (Spike Protein)

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Spike RBD RAS-T032 Anti-SARS-CoV-2 (P.1) Antibody IgG Titer Serologic Assay kit (Spike RBD)

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Spike protein RAS-T033 Anti-SARS-CoV-2 (P.1) Antibody IgG Titer Serologic Assay kit (Spike Protein)

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Spike RBD RAS-T014 Anti-SARS-CoV-2 Antibody IgG1 Titer Serologic Assay kit

订购

Spike RBD RAS-T015 Anti-SARS-CoV-2 Antibody IgG2 Titer Serologic Assay kit

订购

Spike RBD RAS-T016 Anti-SARS-CoV-2 Antibody IgG3 Titer Serologic Assay kit

订购

Spike RBD RAS-T017 Anti-SARS-CoV-2 Antibody IgG4 Titer Serologic Assay kit

订购

抗原检测试剂盒 Nucleocapsid Protein RAS-A010 SARS-CoV-2 Nucleocapsid Protein Titer Assay Kit

订购

Spike protein RAS-A020 SARS-CoV-2 Spike Protein Titer Assay Kit

订购

Spike RBD RAS-A021 SARS-CoV-2 Spike RBD Titer Assay Kit

订购

抑制剂筛选试剂盒 Spike protein EP-105 SARS-CoV-2 (COVID-19) Inhibitor screening Kit

订购

抗原预包被酶标板 S1 protein SP-12 SARS-CoV-2 Spike S1 protein-Coated Plates, Clear, 96-Well

订购

S protein RBD RP-13 SARS-CoV-2 Spike protein RBD-Coated Plates, Clear, 96-Well

订购

抗体类
分子 货号 产品名称
S protein RBD SPD-M180 Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody (AM180)
SPD-M128 Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128)
S1 protein S1N-M122 Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122)
S1N-M130 Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130)
抗体亚型检测原料
类别 货号 产品名称
抗体亚型 SAD-S35 Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1(AS35)
SPD-S66 Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG2(AS35)
SPD-S67 Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG3(AS35)
SPD-S68 Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG4(AS35)
HRP标记二抗 IG1-S71 Mouse Anti-Human IgG1 secondary antibody, Mouse IgG1, HRP
IG2-S72 Mouse Anti-Human IgG2 secondary antibody, Mouse IgG1, HRP
IG3-S73 Mouse Anti-Human IgG3 secondary antibody, Mouse IgG1, HRP
IG4-S74 Mouse Anti-Human IgG4 secondary antibody, Mouse IgG1, HRP
参考文献:
[1] Mulligan et al., (2020). Phase 1/2 Study to Describe the Safety and Immunogenicity of a COVID-19 RNA Vaccine Candidate (BNT162b1) in Adults 18 to 55 Years of Age: Interim Report. medRxiv, doi: https://doi.org/10.1101/2020.06.30.20142570 )

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