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Integrin(整合素)系列蛋白

背景
Integrin是由α(120~185kD)和β(90~110kD)两个亚单位形成的异二聚体跨膜糖蛋白,迄今已发现约18种α亚单位和8种β亚单位,它们按不同的组合构成20余种功能不同的Integrin分子。Integrin作为一类具有信号转导功能的细胞表面粘附分子,几乎在肿瘤转移的每一步都发挥着重要的作用,参与介导肿瘤细胞与细胞外基质(ECM)、肿瘤细胞与宿主细胞间的相互作用,对肿瘤的生长、侵袭、转移等过程产生着影响。
为满足市场需求,ACROBiosystems已经开发了17种由HEK293表达的Integrin分子蛋白,ACRO提供的Integrin系列蛋白适用于大分子抗体药物的免疫、SPR、BLI、细胞活性检测等实验,更有AviTag生物素标记的Integrin蛋白可以助力于您的药物开发。 ACRO对Integrin系列蛋白的纯度、活性等性能进行了验证,并将研发人员实际的实验操作和参数整理成了相应的protocol,ACRO免费提供protocol,可以帮助您节省研发周期哦~
应用
Integrin蛋白应用
产品特点
Integrin蛋白由高质量的HEK293细胞表达
Integrin蛋白由高质量的HEK293细胞表达
高生物活性
SPR:
Integrin SPR

Human MAdCAM-1, His Tag (Cat. No. MAM-H52H4) immobilized on CM5 Chip can bind Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W4) with an affinity constant of 0.227 μM as determined in a SPR assay (Biacore 8K) (routinely tested).

Protocol

SPR:
Integrin SPR

Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283L) immobilized on CM5 Chip can bind Human Integrin alpha 6 beta 1 (ITGA6&ITGB1) Heterodimer Protein, His Tag&Tag Free with an affinity constant between 1.00 nM - 15.0 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol

ELISA:
Integrin ELISA

Immobilized Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W6) with a linear range of 0.039-0.625 μg/mL (QC tested)

Protocol

Integrin ELISA

Immobilized Mouse Latent TGFB1, His Tag (Cat. No. TG1-M5245) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT6-M82W9) with a linear range of 1-20 ng/mL (QC tested)

Protocol

高批间一致性
Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV&ITGB1 Heterodimer Protein, His Tag & Tag Free (Cat. No. IT1-H52E1) with a linear range of 0.02-0.313 μg/mL.

*如果您希望ACRO补充更多Integrin产品的实验设计和应用数据,请 点击咨询

*如果您对ACRO有新品开发建议或者对Integrin蛋白有增项建议,请点击 开发建议

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