Cell based assay
Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) inhibits Anti-CD3-induced proliferation of PBMC. The ED50 for this effect is 0.48-0.57 μg/mL.
Protocol
BLI
Loaded Anti-Siglec-15 MAb (Mouse IgG) on AMC Biosensor, can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 59.7 pM as determined in BLI assay (ForteBio Octet Red96e).
Protocol
SPR
Immobilized Neu5Ac(a2-6)GalNAc-PAA-biotin on SA Chip can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 4.49 μM as determined in a SPR assay (Biacore T200).
Protocol
ELISA
Immobilized Mouse Human chimeric MAb (5G12, Human IgG1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) with a linear range of 0.4-3 ng/mL.
Protocol
Cat. No. | Species | Product Description |
SG5-H5253 | Human | Human Siglec-15 / CD33L3 Protein, Fc Tag |
SG5-H5254 | Human | Human Siglec-15 / CD33L3 Protein, Mouse IgG2a Fc Tag (MALS verified) |
SG5-H52H3 | Human | Human Siglec-15 / CD33L3 Protein, His Tag (MALS verified) |
SG5-H82E9 | Human | Biotinylated Human Siglec-15 / CD33L3 Protein, His, (recommended for biopanning) |
SG5-H82F5 | Human | Biotinylated Human Siglec-15 / CD33L3 Protein, Fc, |
SG5-M52H7 | Mouse | Mouse Siglec-15 / CD33L3 Protein, His Tag |
SG5-C5253 | Rhesus macaque | Rhesus macaque Siglec-15 / CD33L3 Protein, Fc Tag |
SG5-C52H6 | Rhesus macaque | Rhesus macaque Siglec-15 / CD33L3 Protein, His Tag |
ACROBiosystems百普赛斯生物科技股份有限公司
北京:010-53681107
上海:021-50850665
邮箱:(产品订购)order.cn@acrobiosystems.com
邮箱:(技术支持)tech.cn@acrobiosystems.com
邮箱:(廉洁合规)lianjie@acrobiosystems.com
地址:北京经济技术开发区宏达北路8号5号楼4层