背景
癌细胞表面表达特定的“别吃我”的信号分子(如CD24、CD47、PD-L1、B2M)后会被免疫细胞识别为正常细胞,从而逃避肿瘤微环境中巨噬细胞的吞噬清除。最早发现的“别吃我”信号蛋白是PD-1和CTLA-4,2019年7月发表在Nature的一项研究表明CD24是卵巢癌和三阴乳腺癌细胞表达的另一个“别吃我”信号蛋白,被癌细胞用来保护自己,CD24是开发癌症免疫疗法潜在靶点。
CD24(Cluster of Differentiation 24)是一种高度糖基化的糖基磷脂酰肌醇锚定的细胞表面蛋白。卵巢癌和三阴乳腺癌细胞表面上表达的CD24蛋白可以与免疫细胞上的Siglec-10蛋白相互作用来传递免疫抑制性信号,从而抑制破坏性炎症反应。
目前,市面的CD24蛋白大多都只有与抗体结合的数据,缺少与Siglec-10蛋白结合活性的验证,而CD24抗体药研发的关键点在于其能够有效地阻断CD24与Siglec-10的结合。为了解决这些问题,ACROBiosystems开发了不同标签的CD24蛋白以及CD24过表达细胞株,并验证了细胞株与Siglec-10的结合活性。另外还验证了抗CD24中和抗体SN3可以有效的阻断Siglec-10与CD24过表达细胞株的结合。
产品列表
Molecule
|
Cat. No.
|
Species
|
Product Description
|
CD24 | CD4-H82E9 | Human | Biotinylated Human CD24 Protein, His,Avitag™ |
CD4-H82F5 | Human | Biotinylated Human CD24 Protein, Fc,Avitag™ |
CD4-H5254 | Human | Human CD24 Protein, Fc Tag |
CD4-H5257 | Human | Human CD24 Protein, Mouse IgG2a Fc Tag |
CD4-H52H3 | Human | Human CD24 Protein, His Tag |
CD4-M52H7 | Mouse | Mouse CD24 Protein, His Tag |
CD4-C8254 | Cynomolgus | Biotinylated Cynomolgus CD24 Protein, Fc Tag, ultra sensitivity (primary amine labeling) |
CD4-C5257 | Cynomolgus | Cynomolgus CD24 Protein, Fc Tag |
Siglec-10 | SI0-HP2E5 | Human | PE-Labeled Human Siglec-10 Protein, His Tag (recommended for neutralizing assay)HOT |
SI0-H82E3 | Human | Biotinylated Human Siglec-10 Protein, His,Avitag™ |
SI0-H82F6 | Human | Biotinylated Human Siglec-10 Protein, Fc,Avitag™ |
SI0-H5253 | Human | Human Siglec-10 Protein, Fc Tag |
SI0-H525b | Human | Human Siglec-10 Protein, Mouse IgG2a Fc Tag, low endotoxin |
SI0-H52H9 | Human | Human Siglec-10 Protein, His Tag |
SI0-M52H7 | Mouse | Mouse Siglec-10 Protein, His Tag |
SI0-C52H5 | Cynomolgus | Cynomolgus Siglec-10 Protein, His Tag |
实验数据
CD24过表达细胞株与Siglec-10的结合验证
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PE Labeled Siglec-10 Protein
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Fig.1 FACS analysis of PE-Labeled Human Siglec-10, His Tag (Cat. No. SI0-HP2E5) at 1/10 dilution binding to 293 cells overexpressing Human CD24 Stable Cell Line (Cat. No. CHEK-ATP032).
抗CD24抗体(SN3)抑制Siglec-10与CD24过表达细胞株的结合
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Inhibition of Anti-CD24 Antibody (SN3)
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Fig.2 FACS analysis shows that the binding of PE-Labeled Human Siglec-10, His Tag (Cat. No. SI0-HP2E5) to 293 cells overexpressing Human CD24 Stable Cell Line (Cat. No. CHEK-ATP032) was inhibited by increasing concentration of neutralizing anti-CD24 antibody [SN3]. The IC50 of neutralizing anti-CD24 antibody [SN3] is 3.026 μg/ml.
相关研究进展
CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy.
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