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Human Frizzled-2 / FZD2 (24-190) Protein, Fc Tag

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分子别名(Synonym)

FZD2,Frizzled-2,Fz-2,hFz2,FzE2

表达区间及表达系统(Source)

Human Frizzled-2, Fc Tag (FZ2-H5255) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Pro 190 (Accession # Q14332-1).

Predicted N-terminus: Gln 24

Request for sequence

蛋白结构(Molecular Characterization)

Frizzled-2 Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 44.4 kDa. The protein migrates as 50-62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Frizzled-2 SDS-PAGE

Human Frizzled-2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Frizzled-2 ELISA

Immobilized Human Frizzled-2, Fc Tag (Cat. No. FZ2-H5255) at 1 μg/mL (100 μL/well) can bind Wnt surrogate Human IgG1(D239E,L241M) | Human Lambda with a linear range of 0.1-8 ng/mL (QC tested).

Protocol

 

背景(Background)

Frizzled-2 (FZD2) is also known as FzE2, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD2 contains one FZ (frizzled) domain. FZD2 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. The Lys-Thr-X-X-X-Trp motif of FZD2 interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.

文献引用(Citations)

 

前沿进展

 
 
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Frizzled-2靶点信息
英文全称:Frizzled-2
中文全称:卷曲蛋白-2
种类:
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床一期
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