膜杰作
Star Staining
分子别名（Synonym）
MSLN,Mesothelin,MPF
表达区间及表达系统（Source）
FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF223) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272). It is the FITC labeled form of Human Mesothelin (296-580), His Tag.
Predicted N-terminus: Glu 296
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
蛋白标记度（Protein Ratio）
The FITC to protein molar ratio is 0.5-1.5.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
FITC-Labeled Human Mesothelin (296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Anti-Human MSLN MAb at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF223) with a linear range of 0.078-0.313 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).
Protocol
CAR阳性表达率检测（Evaluation of CAR expression）
FACS Analysis of Anti-MSLN CAR Expression
293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF223, 1 μg/mL) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF223).
背景（Background）
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
