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 >  Protein>Syndecan-1 >SD1-HF228

FITC-Labeled Human Syndecan-1 Protein, His Tag

分子别名(Synonym)

SDC1,Syndecan-1,CD138,SYND1,SDC

表达区间及表达系统(Source)

FITC-Labeled Human Syndecan-1, His Tag (SD1-HF228) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 254 (Accession # P18827-1).

Predicted N-terminus: Gln 23

Request for sequence

蛋白结构(Molecular Characterization)

Syndecan-1 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 25.8 kDa. The protein migrates as 42-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(Protein Ratio)

The FITC to protein molar ratio is 1.5-3.5.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Syndecan-1 SDS-PAGE

FITC-Labeled Human Syndecan-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Syndecan-1 ELISA

Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human Syndecan-1, His Tag (Cat. No. SD1-HF228) with a linear range of 0.078-2.5 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-FACS

Syndecan-1 FACS

2e5 of anti-Syndecan-1 CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human Syndecan-1, His Tag (Cat. No. SD1-HF228) and negative control protein respectively, FITC signals was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.

 

前沿进展

 
 
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Syndecan-1靶点信息
英文全称:Syndecan-1
中文全称:多配体蛋白聚糖-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
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