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 >  Protein>TGF-beta RII >TG2-M82H5

Biotinylated Mouse TGF-beta RII / TGFBR2 Protein, His,Avitag™

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分子别名(Synonym)

TGFBR2,TGFR2,TbetaR-II,TGFβR2

表达区间及表达系统(Source)

Biotinylated Mouse TGF-beta RII, His,Avitag (TG2-M82H5) is expressed from human 293 cells (HEK293). It contains AA Ile 24 - Asp 184 (Accession # Q62312-1).

Predicted N-terminus: Ile 24

Request for sequence

蛋白结构(Molecular Characterization)

TGF-beta RII Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 21.7 kDa. The protein migrates as 32-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

TGF-beta RII SDS-PAGE

Biotinylated Mouse TGF-beta RII, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

TGF-beta RII ELISA

Immobilized Human Latent TGFB1, His Tag (Cat. No. TG1-H524x) at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse TGF-beta RII, His,Avitag (Cat. No. TG2-M82H5) with a linear range of 1-31 ng/mL (QC tested).

Protocol

 

背景(Background)

TGF-beta receptor type-2 (TGFBR2 or TGFR-2) is also known as TGF-beta type II receptor, Transforming growth factor-beta receptor type II, TbetaR-II, TGFβR2, which is a homodimer or heterohexamer, belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family and TGFB receptor subfamily. TGFR2 / TGFBR2 binds TGF-β1 / TGFB1 and TGF-β3 / TGFB3 with high affinity and TGF-β2 / TGFB2 with a much lower affinity. This type I I receptor forms a heterodimeric complex with type I receptor and is essential for signal transduction. Upon ligand binding, the TGFR2 autophosphorylates its cytoplasmic domain and subsequently phosphorylates the downstream molecules which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation.

 

前沿进展

 
 
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TGF-beta RII靶点信息
英文全称:Transforming growth factor beta receptor 2
中文全称:转化生长因子β受体2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
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