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 >  Protein>Cadherin-17 >CA7-H52H3

Human Cadherin-17 / CDH17 Protein, His Tag

分子别名(Synonym)

Cadherin-17,CDH17,HPT-1,LI-cadherin

表达区间及表达系统(Source)

Human Cadherin-17, His Tag (CA7-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Met 787 (Accession # Q12864).

Predicted N-terminus: Gln 23

Request for sequence

蛋白结构(Molecular Characterization)

Cadherin-17 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 86.9 kDa. The protein migrates as 95-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150mM NaCl, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Cadherin-17 SDS-PAGE

Human Cadherin-17, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

Cadherin-17 ELISA

Immobilized Human Cadherin-17, His Tag (Cat. No. CA7-H52H3) at 1 μg/mL (100 μL/well) can bind Anti-Cadherin 17/CDH17 Antibody with a linear range of 0.0000122-0.000391 % (QC tested).

Protocol

 

活性(Bioactivity)-SPR

Cadherin-17 SPR

Anti-Cadherin-17 antibody captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human Cadherin-17, His Tag (Cat. No. CA7-H52H3) with an affinity constant of 2.9 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.

 

前沿进展

 
 
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Cadherin-17靶点信息
英文全称:Cadherin-17
中文全称:钙粘蛋白17
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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